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ii real time polymerase chain reaction pcr detection kit  (Bio-Rad)


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    Structured Review

    Bio-Rad ii real time polymerase chain reaction pcr detection kit
    Ii Real Time Polymerase Chain Reaction Pcr Detection Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ii+real+time+polymerase+chain+reaction+pcr+detection+kit/iQ-Check+Listeria+monocytogenes+II+PCR+Detection+Kit/10__1016_slash_j__foodcont__2026__112090-74-4-12
    Average 93 stars, based on 24 article reviews
    ii real time polymerase chain reaction pcr detection kit - by Bioz Stars, 2026-11
    93/100 stars

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    Real-time Polymerase Chain Reaction:

    Article Title: Listeria monocytogenes from salmon processing and ready-to-eat seafood: Antibiotic susceptibility and disinfectant tolerance
    Article Snippet: .. The iQ-Check Listeria monocytogenes II real-time polymerase chain reaction (PCR) Detection Kit (Bio-Rad, Hercules, USA) was used to confirm the presence of L. monocytogenes in swab samples following the manufacturer's protocol, including enrichment, DNA extraction, and PCR. .. In brief, swabs were homogenized in Listeria Special Broth (LSB) (Bio-Rad, Hercules, USA) and incubated at 37 ◦C for 16 – 24 h for enrichment.

    Polymerase Chain Reaction:

    Article Title: Listeria monocytogenes from salmon processing and ready-to-eat seafood: Antibiotic susceptibility and disinfectant tolerance
    Article Snippet: .. The iQ-Check Listeria monocytogenes II real-time polymerase chain reaction (PCR) Detection Kit (Bio-Rad, Hercules, USA) was used to confirm the presence of L. monocytogenes in swab samples following the manufacturer's protocol, including enrichment, DNA extraction, and PCR. .. In brief, swabs were homogenized in Listeria Special Broth (LSB) (Bio-Rad, Hercules, USA) and incubated at 37 ◦C for 16 – 24 h for enrichment.

    DNA Extraction:

    Article Title: Listeria monocytogenes from salmon processing and ready-to-eat seafood: Antibiotic susceptibility and disinfectant tolerance
    Article Snippet: .. The iQ-Check Listeria monocytogenes II real-time polymerase chain reaction (PCR) Detection Kit (Bio-Rad, Hercules, USA) was used to confirm the presence of L. monocytogenes in swab samples following the manufacturer's protocol, including enrichment, DNA extraction, and PCR. .. In brief, swabs were homogenized in Listeria Special Broth (LSB) (Bio-Rad, Hercules, USA) and incubated at 37 ◦C for 16 – 24 h for enrichment.



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    Figure 4. Expression of Bak, Bax and Caspase‑3 and activity of Caspase‑3 in MDA‑MB‑468 cells. Reverse transcription‑quantitative <t>polymerase</t> <t>chain</t> <t>reaction</t> analysis of the relative gene expression of (A) Bak and (B) Caspase‑3 in MDA‑MB‑468 cells following wt‑hTRβ1 or m‑hTRβ1‑gene transfection. The expression levels were normalized to those of GAPDH; the relative expression level of each gene was calculated using 2‑∆∆Cq. The data are presented as the mean ± SD. (C) Western blot analysis of the effect of wt‑hTRβ1 and m‑hTRβ1 on the expression of Bak and Caspase‑3. The results are from a single experiment that is representative of three independent experiments. (D) Reverse transcription‑quantitative polymerase chain reaction analysis of the relative gene expres sion of Bax in MDA‑MB‑468 cells following wt‑hTRβ1 or m‑hTRβ1‑gene transfection. (E) Colorimetric assay of Caspase‑3 activation. Data are presented as the mean ± SD. *P<0.05; **P<0.01. wt‑hTRβ1, wild‑type human TRβ1; m‑hTRβ1, modified human TRβ1; NNS, nucleic kinase substrate short peptide; CS, chitosan; T3, triiodothyronine; SD, standard deviation.
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    Figure 4. Expression of Bak, Bax and Caspase‑3 and activity of Caspase‑3 in MDA‑MB‑468 cells. Reverse transcription‑quantitative polymerase chain reaction analysis of the relative gene expression of (A) Bak and (B) Caspase‑3 in MDA‑MB‑468 cells following wt‑hTRβ1 or m‑hTRβ1‑gene transfection. The expression levels were normalized to those of GAPDH; the relative expression level of each gene was calculated using 2‑∆∆Cq. The data are presented as the mean ± SD. (C) Western blot analysis of the effect of wt‑hTRβ1 and m‑hTRβ1 on the expression of Bak and Caspase‑3. The results are from a single experiment that is representative of three independent experiments. (D) Reverse transcription‑quantitative polymerase chain reaction analysis of the relative gene expres sion of Bax in MDA‑MB‑468 cells following wt‑hTRβ1 or m‑hTRβ1‑gene transfection. (E) Colorimetric assay of Caspase‑3 activation. Data are presented as the mean ± SD. *P<0.05; **P<0.01. wt‑hTRβ1, wild‑type human TRβ1; m‑hTRβ1, modified human TRβ1; NNS, nucleic kinase substrate short peptide; CS, chitosan; T3, triiodothyronine; SD, standard deviation.

    Journal: Oncology letters

    Article Title: Overexpressing modified human TRβ1 suppresses the proliferation of breast cancer MDA-MB-468 cells.

    doi: 10.3892/ol.2018.8764

    Figure Lengend Snippet: Figure 4. Expression of Bak, Bax and Caspase‑3 and activity of Caspase‑3 in MDA‑MB‑468 cells. Reverse transcription‑quantitative polymerase chain reaction analysis of the relative gene expression of (A) Bak and (B) Caspase‑3 in MDA‑MB‑468 cells following wt‑hTRβ1 or m‑hTRβ1‑gene transfection. The expression levels were normalized to those of GAPDH; the relative expression level of each gene was calculated using 2‑∆∆Cq. The data are presented as the mean ± SD. (C) Western blot analysis of the effect of wt‑hTRβ1 and m‑hTRβ1 on the expression of Bak and Caspase‑3. The results are from a single experiment that is representative of three independent experiments. (D) Reverse transcription‑quantitative polymerase chain reaction analysis of the relative gene expres sion of Bax in MDA‑MB‑468 cells following wt‑hTRβ1 or m‑hTRβ1‑gene transfection. (E) Colorimetric assay of Caspase‑3 activation. Data are presented as the mean ± SD. *P<0.05; **P<0.01. wt‑hTRβ1, wild‑type human TRβ1; m‑hTRβ1, modified human TRβ1; NNS, nucleic kinase substrate short peptide; CS, chitosan; T3, triiodothyronine; SD, standard deviation.

    Article Snippet: M-MLV reverse transcriptase, TRIzol® total RNA extraction reagent and quantitative real-time polymerase chain reaction (PCR) detection kits (SYBR® Premix Ex TaqTM II; cat. no. RR820B were obtained from Takara Bio, Inc. (Otsu, Japan).

    Techniques: Expressing, Activity Assay, Polymerase Chain Reaction, Gene Expression, Transfection, Western Blot, Colorimetric Assay, Activation Assay, Modification, Standard Deviation